p atf2 Search Results


primarymouse antibody against patf2  (Santa Cruz Biotechnology)
93
Santa Cruz Biotechnology primarymouse antibody against patf2
Primarymouse Antibody Against Patf2, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/primarymouse antibody against patf2/product/Santa Cruz Biotechnology
Average 93 stars, based on 1 article reviews
primarymouse antibody against patf2 - by Bioz Stars, 2026-05
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p-atf2 ser62 primary antibody  (Abnova)
90
Abnova p-atf2 ser62 primary antibody
Pak1 Phosphorylates <t>ATF2</t> on <t>Ser62</t> and Inhibits ATF2 Translocation into the Cell Nucleus (A) BGC823 cells were transfected with Pak1-RNAi lentivirus, and the location of ATF2 was analyzed by confocal imaging. (B) FLAG-ATF2 and myc-Pak1 plasmids were co-transfected into BGC823 cells, cell lysates were subjected to immunoprecipitation with myc-specific antibody, and the western blot was probed with FLAG-specific antibody. (C) BGC823 cell lysates were immunoprecipitated with Pak1-specific antibody or IgG and then analyzed by western blot with ATF2 antibody. (D) Kinase assays were performed with purified ATF2 and ATF2S62A protein as substrates for Pak1 kinase. (E) The location of p-ATF2 was examined by confocal imaging assay; p-ATF2 is marked with red and nuclear DNA is marked with blue (top panels). Location of ATF2 and p-ATF2 in paraffin sections of tissues from two gastric cancer patients were evaluated by immunohistochemistry (bottom panels). (F) BGC823 cells were transfected with ATF2WT and ATF2S62A plasmids, and confocal imaging was performed. (G) BGC823 cells were transfected with Pak1-RNAi lentivirus, and protein in cytoplasmic and nuclear extracts was prepared and analyzed by western blot. β-Tubulin and PARP served as cytoplasmic and nuclear protein loading controls, respectively. (H) BGC823 cells were transfected with ATF2WT and ATF2S62A plasmids, and protein in the cytoplasm and nucleus was detected with western blot. All experiments were carried out as described in .
P Atf2 Ser62 Primary Antibody, supplied by Abnova, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/p-atf2 ser62 primary antibody/product/Abnova
Average 90 stars, based on 1 article reviews
p-atf2 ser62 primary antibody - by Bioz Stars, 2026-05
90/100 stars
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anti p atf2  (Novus Biologicals)
91
Novus Biologicals anti p atf2
Pak1 Phosphorylates <t>ATF2</t> on <t>Ser62</t> and Inhibits ATF2 Translocation into the Cell Nucleus (A) BGC823 cells were transfected with Pak1-RNAi lentivirus, and the location of ATF2 was analyzed by confocal imaging. (B) FLAG-ATF2 and myc-Pak1 plasmids were co-transfected into BGC823 cells, cell lysates were subjected to immunoprecipitation with myc-specific antibody, and the western blot was probed with FLAG-specific antibody. (C) BGC823 cell lysates were immunoprecipitated with Pak1-specific antibody or IgG and then analyzed by western blot with ATF2 antibody. (D) Kinase assays were performed with purified ATF2 and ATF2S62A protein as substrates for Pak1 kinase. (E) The location of p-ATF2 was examined by confocal imaging assay; p-ATF2 is marked with red and nuclear DNA is marked with blue (top panels). Location of ATF2 and p-ATF2 in paraffin sections of tissues from two gastric cancer patients were evaluated by immunohistochemistry (bottom panels). (F) BGC823 cells were transfected with ATF2WT and ATF2S62A plasmids, and confocal imaging was performed. (G) BGC823 cells were transfected with Pak1-RNAi lentivirus, and protein in cytoplasmic and nuclear extracts was prepared and analyzed by western blot. β-Tubulin and PARP served as cytoplasmic and nuclear protein loading controls, respectively. (H) BGC823 cells were transfected with ATF2WT and ATF2S62A plasmids, and protein in the cytoplasm and nucleus was detected with western blot. All experiments were carried out as described in .
Anti P Atf2, supplied by Novus Biologicals, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/anti p atf2/product/Novus Biologicals
Average 91 stars, based on 1 article reviews
anti p atf2 - by Bioz Stars, 2026-05
91/100 stars
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antibody against p atf2  (Novus Biologicals)
88
Novus Biologicals antibody against p atf2
Pak1 Phosphorylates <t>ATF2</t> on <t>Ser62</t> and Inhibits ATF2 Translocation into the Cell Nucleus (A) BGC823 cells were transfected with Pak1-RNAi lentivirus, and the location of ATF2 was analyzed by confocal imaging. (B) FLAG-ATF2 and myc-Pak1 plasmids were co-transfected into BGC823 cells, cell lysates were subjected to immunoprecipitation with myc-specific antibody, and the western blot was probed with FLAG-specific antibody. (C) BGC823 cell lysates were immunoprecipitated with Pak1-specific antibody or IgG and then analyzed by western blot with ATF2 antibody. (D) Kinase assays were performed with purified ATF2 and ATF2S62A protein as substrates for Pak1 kinase. (E) The location of p-ATF2 was examined by confocal imaging assay; p-ATF2 is marked with red and nuclear DNA is marked with blue (top panels). Location of ATF2 and p-ATF2 in paraffin sections of tissues from two gastric cancer patients were evaluated by immunohistochemistry (bottom panels). (F) BGC823 cells were transfected with ATF2WT and ATF2S62A plasmids, and confocal imaging was performed. (G) BGC823 cells were transfected with Pak1-RNAi lentivirus, and protein in cytoplasmic and nuclear extracts was prepared and analyzed by western blot. β-Tubulin and PARP served as cytoplasmic and nuclear protein loading controls, respectively. (H) BGC823 cells were transfected with ATF2WT and ATF2S62A plasmids, and protein in the cytoplasm and nucleus was detected with western blot. All experiments were carried out as described in .
Antibody Against P Atf2, supplied by Novus Biologicals, used in various techniques. Bioz Stars score: 88/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/antibody against p atf2/product/Novus Biologicals
Average 88 stars, based on 1 article reviews
antibody against p atf2 - by Bioz Stars, 2026-05
88/100 stars
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p atf2  (Cell Signaling Technology Inc)
86
Cell Signaling Technology Inc p atf2
Pak1 Phosphorylates <t>ATF2</t> on <t>Ser62</t> and Inhibits ATF2 Translocation into the Cell Nucleus (A) BGC823 cells were transfected with Pak1-RNAi lentivirus, and the location of ATF2 was analyzed by confocal imaging. (B) FLAG-ATF2 and myc-Pak1 plasmids were co-transfected into BGC823 cells, cell lysates were subjected to immunoprecipitation with myc-specific antibody, and the western blot was probed with FLAG-specific antibody. (C) BGC823 cell lysates were immunoprecipitated with Pak1-specific antibody or IgG and then analyzed by western blot with ATF2 antibody. (D) Kinase assays were performed with purified ATF2 and ATF2S62A protein as substrates for Pak1 kinase. (E) The location of p-ATF2 was examined by confocal imaging assay; p-ATF2 is marked with red and nuclear DNA is marked with blue (top panels). Location of ATF2 and p-ATF2 in paraffin sections of tissues from two gastric cancer patients were evaluated by immunohistochemistry (bottom panels). (F) BGC823 cells were transfected with ATF2WT and ATF2S62A plasmids, and confocal imaging was performed. (G) BGC823 cells were transfected with Pak1-RNAi lentivirus, and protein in cytoplasmic and nuclear extracts was prepared and analyzed by western blot. β-Tubulin and PARP served as cytoplasmic and nuclear protein loading controls, respectively. (H) BGC823 cells were transfected with ATF2WT and ATF2S62A plasmids, and protein in the cytoplasm and nucleus was detected with western blot. All experiments were carried out as described in .
P Atf2, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/p atf2/product/Cell Signaling Technology Inc
Average 86 stars, based on 1 article reviews
p atf2 - by Bioz Stars, 2026-05
86/100 stars
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atf2 [p thr52] antibody  (Novus Biologicals)
N/A
The ATF2 [p Thr52] Antibody from Novus is a ATF2 antibody to ATF2. This antibody reacts with Human, Mouse. The ATF2 antibody has been validated for the following applications: Western Blot.
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atf2 [p thr71] antibody (103c411.2) - azide free  (Novus Biologicals)
N/A
The ATF2 [p Thr71] Antibody (103C411.2) - Azide Free from Novus is a ATF2 antibody to ATF2. This antibody reacts with Human. The ATF2 antibody has been validated for the following applications: Western Blot, Flow
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atf2 [p thr71] antibody (103c411.2) [alexa fluor® 350]  (Novus Biologicals)
N/A
The ATF2 [p Thr71] Antibody (103C411.2) [Alexa Fluor® 350] from Novus is a ATF2 antibody to ATF2. This antibody reacts with Human. The ATF2 antibody has been validated for the following applications: Western Blot, Flow
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atf2 [p thr71] antibody (103c411.2) [dylight 488]  (Novus Biologicals)
N/A
The ATF2 [p Thr71] Antibody (103C411.2) [DyLight 488] from Novus is a ATF2 antibody to ATF2. This antibody reacts with Human. The ATF2 antibody has been validated for the following applications: Western Blot, Flow Cytometry.
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atf2 [p thr71] antibody (103c411.2) [janelia fluor® 646]  (Novus Biologicals)
N/A
The ATF2 [p Thr71] Antibody (103C411.2) [Janelia Fluor® 646] from Novus is a ATF2 antibody to ATF2. This antibody reacts with Human. The ATF2 antibody has been validated for the following applications: Western Blot, Flow
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atf2 [p thr71] antibody (103c411.2) [percp]  (Novus Biologicals)
N/A
The ATF2 [p Thr71] Antibody (103C411.2) [PerCP] from Novus is a ATF2 antibody to ATF2. This antibody reacts with Human. The ATF2 antibody has been validated for the following applications: Western Blot, Flow Cytometry.
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Image Search Results


Pak1 Phosphorylates ATF2 on Ser62 and Inhibits ATF2 Translocation into the Cell Nucleus (A) BGC823 cells were transfected with Pak1-RNAi lentivirus, and the location of ATF2 was analyzed by confocal imaging. (B) FLAG-ATF2 and myc-Pak1 plasmids were co-transfected into BGC823 cells, cell lysates were subjected to immunoprecipitation with myc-specific antibody, and the western blot was probed with FLAG-specific antibody. (C) BGC823 cell lysates were immunoprecipitated with Pak1-specific antibody or IgG and then analyzed by western blot with ATF2 antibody. (D) Kinase assays were performed with purified ATF2 and ATF2S62A protein as substrates for Pak1 kinase. (E) The location of p-ATF2 was examined by confocal imaging assay; p-ATF2 is marked with red and nuclear DNA is marked with blue (top panels). Location of ATF2 and p-ATF2 in paraffin sections of tissues from two gastric cancer patients were evaluated by immunohistochemistry (bottom panels). (F) BGC823 cells were transfected with ATF2WT and ATF2S62A plasmids, and confocal imaging was performed. (G) BGC823 cells were transfected with Pak1-RNAi lentivirus, and protein in cytoplasmic and nuclear extracts was prepared and analyzed by western blot. β-Tubulin and PARP served as cytoplasmic and nuclear protein loading controls, respectively. (H) BGC823 cells were transfected with ATF2WT and ATF2S62A plasmids, and protein in the cytoplasm and nucleus was detected with western blot. All experiments were carried out as described in .

Journal: Molecular Therapy. Nucleic Acids

Article Title: A Novel Pak1/ATF2/miR-132 Signaling Axis Is Involved in the Hematogenous Metastasis of Gastric Cancer Cells

doi: 10.1016/j.omtn.2017.07.005

Figure Lengend Snippet: Pak1 Phosphorylates ATF2 on Ser62 and Inhibits ATF2 Translocation into the Cell Nucleus (A) BGC823 cells were transfected with Pak1-RNAi lentivirus, and the location of ATF2 was analyzed by confocal imaging. (B) FLAG-ATF2 and myc-Pak1 plasmids were co-transfected into BGC823 cells, cell lysates were subjected to immunoprecipitation with myc-specific antibody, and the western blot was probed with FLAG-specific antibody. (C) BGC823 cell lysates were immunoprecipitated with Pak1-specific antibody or IgG and then analyzed by western blot with ATF2 antibody. (D) Kinase assays were performed with purified ATF2 and ATF2S62A protein as substrates for Pak1 kinase. (E) The location of p-ATF2 was examined by confocal imaging assay; p-ATF2 is marked with red and nuclear DNA is marked with blue (top panels). Location of ATF2 and p-ATF2 in paraffin sections of tissues from two gastric cancer patients were evaluated by immunohistochemistry (bottom panels). (F) BGC823 cells were transfected with ATF2WT and ATF2S62A plasmids, and confocal imaging was performed. (G) BGC823 cells were transfected with Pak1-RNAi lentivirus, and protein in cytoplasmic and nuclear extracts was prepared and analyzed by western blot. β-Tubulin and PARP served as cytoplasmic and nuclear protein loading controls, respectively. (H) BGC823 cells were transfected with ATF2WT and ATF2S62A plasmids, and protein in the cytoplasm and nucleus was detected with western blot. All experiments were carried out as described in .

Article Snippet: The sections were incubated overnight at 4°C with ATF2 (Cell Signaling Technology) or p-ATF2 Ser62 (Abnova) primary antibody and then detected with the EliVision plus kit and a 3′3-diaminobenzidine (DAB) kit (MaiXin.Bio).

Techniques: Translocation Assay, Transfection, Imaging, Immunoprecipitation, Western Blot, Purification, Immunohistochemistry